Taxonomic and functional metagenomics profiling of Tuwa and Unnai hot springs microbial communities

Hot springs are of great importance due to their unique physicochemical properties. Due selection pressure in this habitat, a diverse microbial community is prevailing and can be analyzed by high throughput sequencing technology. Present study focuses on metagenomic two hot from Gujarat, India namely Tuwa Unnai through both, culturable culture independent approach. Sequence analysis both the water reservoirs depicted higher species richness diversity based various indices. The structure at was distinct dependent factors like temperature, pH, mineral content etc. Enrichment cultivation before metagenome revealed abundance Firmicutes (up 96%) representing cultivable organisms hotsprings. bacterial phyla Firmicutes, Proteobacteria, Bacteroidetes, Thermotogae, Deinococcus-Thermus, Chloroflexi dominate thermoalkaline spring different proportion. Economically important microorganisms belonging genera Thermus, Brevibacillus, Anoxybacillus, Bacillus, Pseudomonas, Geobacillus were prevalent springs. functional potential KEGG pathways for metabolism carbohydrates, amino acids, vitamins, cofactors xenobiotics. Annotation with Carbohydrate Active EnZymes (CAZy) presence four major classes enzymes: glycosyl transferase, glycoside hydrolase, polysaccharide lyase carbohydrate-binding modules. provides insight into untapped biotechnological environmental applications.